Contents:

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  1. Introduction
    1. Chaetopterus as a model system in developmental biology
      1. oocyte maturation and cell cycle
      2. fertilization
      3. egg organization
      4. regeneration

    2. Early development

  2. Methods for obtaining and using eggs and embryos
    1. Handling adults
    2. Obtaining gametes
      1. eggs
      2. sperm

    3. Oocyte maturation
      1. obtaining oocytes arrested at the G2/M border

    4. Fertilization and embryo culture
      1. insemination procedures
      2. vitelline envelope removal
      3. egg activation
      4. embryo culture

    5. Differentiation without cleavage
    6. Microscopy and micromanipulation
  3. Cytoskeleton
  4. Regeneration
  5. Acknowledgments
  6. Literature Cited
  7. Appendices
  8. Figures