TABLE 8.   BUFFERED SALINES - VERTEBRATES*

SELECT A SUBDIVISION:

Table 8A: PHOSPHATE-BUFFERED SALINES

Table 8B: TRIS-BUFFERED SALINES

Table 8C: HEPES-BUFFERED SALINES

*Abbreviations used in table 8.

GENERAL NOTES:
(1) Solutions containing organic components should be refrigerated to discourage bacterial growth.
(2) For uniformity and viewing ease, concentrations are given in mM to two decimal places. However, it is unlikely that there are more than three significant figures in most cases.

TABLE 8A. PHOSPHATE-BUFFERED SALINES

Values shown = millimolar (mM) concentration = ml 1M stock solution per liter.

To determine grams per liter if not shown, see Calculations (App. 1) and Formula Weights (App. 2).

    Abbrev.-->
Ingred.		 PBS-1
(Dulbecco..)		 PBS-2
(Galfre..)		 PBS-3
(Kuchler..)		 PBS-4
(Ausubel..)
NaCl 136.90 136.90 136.90 137.00
KCl 2.68 2.68 4.02 2.70
Na2HPO4 8.10 8.10 5.14 4.30
KH2PO4 1.47 1.47 1.47 1.40
CaCl2 0.90 - - -
MgCl2 0.49 - - -
glucose - - 11.10 -
pH - - 7.8 with NaOH 7.3
System Ma; cell
washes		 Ma; monoclonal
antibody prep.		 Ma; trypsin
disaggregation		 molecular
protocols
Notes N-PBS1 - N-PBS3 -

TABLE 8B. TRIS-BUFFERED SALINES

Values shown for ingredients = millimolar (mM) concentration = ml 1M stock solution per liter
To determine grams per liter, see Calculations (App. 1) and Formula Weights (App. 2).

    Abbrev.-->
Ingred.		 TBS-1
(Goldsmith..)		 TBS-2
(Ausubel..)
NaCl 154.00 136.89
KCl - 5.10
CaCl2 - 1.35
Na2HPO4 - 1.41
TRIS.HCl 100.00 -
TRIS base - 24.76
MgCl2 - 1.05
pH 7.4 7.5
System Ma cell
culture		 molecular
protocols

TABLE 8C. HEPES-BUFFERED SALINES

Values shown for ingredients = millimolar (mM) concentration = ml 1M stock solution per liter
To determine grams per liter, see Calculations (App. 1) and Formula Weights (App. 2).

    Abbrev.-->
Ingred.		 HBS-1
(Dittmar..)		 HBS-2
(Liu..)		 HBS-3
(Ausubel..)
NaCl 150.00 130.00 137.00
KCl - 5.00 5.00
glucose - - 6.00
Na2HPO4 - - 0.70
HEPES 20.00 5.00 21.00
pH 7.4 7.3 7.05
System cultured
Ma cells		 liposomes
(in vitro)		 transfection

Abbreviations used in Table 8:

B = buffered, H = HEPES, Ma = mammalian, P = phosphate, S = saline, T = TRIS

Note: see Appendix 2 for further description of chemical agents (e.g., HEPES, TRIS).