COMPENDIA NOTES, PART A:
ARTIFICIAL SEAWATERS (ASWs)

Notes on the ASWs listed in Table 2 are from ref. [A1] if not otherwise stated.

Directions: Select ASW#

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
21 22


N1 (MBL): According to [A1], this formula was calculated from the Table of Major Constituents of Sea Water [B1] by J.D. Ostrow, and tested at the MBL in 1951 by E.B. Harvey.


N2 (TRIS-MBL): = ASW #1 (MBL ASW) buffered with TRIS [B2].


N3 (CaF-MBL): Formula made isotonic to MBL ASW with NaCl .


N4 (MgF-MBL): Formula made isotonic to MBL ASW with NaCl and Na2SO4.


N5 (CaMgF-MBL): Formula made isotonic to MBL ASW with NaCl, KCl, and Na2SO4.


N6 (Trace-MBL): According to [A1], this formula was calculated from the Table of Major Constituents of Sea Water [B1] by J.D. Ostrow.


N7 (ClF-I-MBL): Chlorides replaced by iodides


N8 (ClF-N-MBL): Chlorides replaced by nitrates


N9 (CaF-M): A calcium-free recipe reported in [A1]; used, for example, with gametes of Pectinaria [B13] Original source not yet determined.


N10 (CaF-S): Original source not yet determined; [A1].


N11 (CaF-E): A calcium-free EGTA-containing formula used with sea anemones [B11].


N12 (NaF): Sodium-free, used with oocytes of the mussel, Mytilus edulis; additional variations are given [B10].


N13 (TES-NaF): A TES-buffered sodium-free formula used in studies of brachiopod larval metamorphosis; many other ASW ionic variations also given [B12].


N14 (TES): A TES-buffered formula used in studies of brachiopod larval metamorphosis; many other ASW ionic variations also given [B12].


N15 (MOPS): A MOPS-buffered formula used to wash marine "worm" (Glycera) erythrocytes [B9]; formula slightly modified from the original appearing in [B9-x].


N16 (BZ): For additional information, see [B3] .


N17 (AP): Original source not yet determined; [A1],[A2].


N18 (HB): For additional information, see [B5].


N19 (HR): For additional information, see [B5].


N20 (VHa): For additional information, see [B6], [B7].


N21 (VHb): For additional information, see [B6], [B8].


N22 (CH): A formulation containing a very high calcium concentration. Original source in the "Challenger Reports" not yet located; [A1].