Cirino et al., Laboratory Culture of the Ascidian Ciona intestinalis(L.):
A Model System For Molecular Developmental Biology Research
Table of Contents:
Title Page
I. Introduction
A. Background
B. Ciona as a model organism
II. Methods for obtaining embryos and larvae
A. Obtaining gametes
1. Eggs
2. Sperm
B. Fertilization procedures and embryo culture
C. Handling larvae
III. Culture of metamorphic and post-metamorphic stages
A. Settlement of larvae
B. Metamorphosis
IV. Experimental setup for culturing juvenile stages
A. Holding apparatus
B. Feeding system
