Click on number or letter of selected section heading.

1. Introduction
2. Collection of adults
3. Seasonal development and natural spawning
4. Maintenance of adults in the laboratory
5. Reproductive system of the adult
6. Ovulation and vitellogenesis in vivo
7. Induced shedding of gametes in vitro
   1. Preparation of adults
   2. Sequence of germinal vesicle breakdown (GVBD)
   3. Fertilization procedures
   4. Timetable of development
8. Induced spawning by maturation factor
9. Experimental procedures
   1. The application of metabolic precursors in vivo and in vitro
   2. Vital dyes and fluorescent tagging of oocytes and early embryos
   3. Stratification of organelles by centrifugation
10. Cytological preparations - in vitro and in vivo
    1. In vitro stages
    2. Preparation of adults for in vivo oocyte studies.
11. Acknowledgments
12. Literature Cited
13. Appendices
14. Figures