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1. Introduction
   1. Erythrocytes of non-mammalian vertebrates
   2. Erythrocytes of the smooth dogfish
2. Background: experimental use of dogfish erythrocytes
   1. Living cells
      1. Temperature-induced disassembly and reassembly of the marginal band of microtubules
      2. Inhibition of marginal band disassembly and of reassembly
      3. Natural marginal band breakage, generating doubly pointed cells
   2. In vitro preparations
      1. Marginal band properties supporting mass isolation
      2. Ability to produce anucleate ghosts and anucleate cytoskeletons
      3. Binding of calmodulin to membrane skeleton alpha-fodrin
      4. Low-temperature marginal band disassembly in vitro, yielding protein that reassembles into microtubule bundles
3. Availability and handling of smooth dogfish at the MBL
   1. Collection and maintenance
   2. Handling smooth dogfish
   3. Smooth vs. spiny dogfish
4. Obtaining blood
5. Separating and washing dogfish erythrocytes
6. Preparing and storing dogfish erythrocyte cytoskeletons
   1. Improved cytoskeleton preparation
   2. Cytoskeleton storage conditions
7. Acknowledgments
8. Literature cited
9. Appendices
10. Figures