TABLE 3. ARTIFICIAL SEAWATERS - RECIPES

Table 3 subdivisions:3A ASWs #1-8: "MBL" Formula ASW and variations
3B ASWs #9-15: Additional Ca++-free and buffer variations
3C ASWs #16-22: Some older ASW vintages

GENERAL NOTES:
(1) All solutions containing organic components (e.g., glucose, urea) should be refrigerated to discourage bacterial growth.
(2) If NaHCO3 is an ingredient together with CaCl2, it should be added last (just before use, if possible) to avoid precipitation of calcium carbonate.
(3) For uniformity and viewing ease, concentrations are given in mM to two decimal places. However, it is unlikely that there are more than three significant figures in most cases.

TABLE 3A.   ASWs #1-8; "MBL" Formula and variations (see KEY)

Values shown = millimolar (mM) concentration = ml 1M stock solution per liter
To determine grams per liter, see Calculations (App. 1) and Formula Weights (App. 2).

         ASW# --->
Ingredients		 1
MBL		 2 TRIS-
MBL		 3 CaF-
MBL		 4 MgF-
MBL		 5 CaMgF-
MBL		 6 Trace-
MBL		 7 ClF-I-
MBL		 8 ClF-N-
MBL
NaCl 423.00 423.00 436.71 481.53 461.85 423.00 - -
KCl 9.00 9.00 9.00 9.00 10.73 8.27 - -
CaCl2 9.27 9.27 - 9.27 - 9.27 - -
MgCl2 22.94 22.94 22.94 - - 22.94 - -
MgSO4 25.50 25.50 25.50 - - 25.50 48.48 25.50
*NaHCO3 2.14 - 2.14 2.14 2.14 2.14 2.14 2.14
H2O to 1L to 1L to 1L to 1L to 1L to 1L to 1L to 1L
Final pH - 8.2 - - - - - -
Other Ingred. 1 2 3 4 5 6 7 8
--->
 - TRIS
5.00		 - Na2SO4
50.96		 Na2SO4
7.04		 SrCl2
0.23		 NaI
422.23		 NaNO3
422.30
--->
 - - - - - NaF
0.07		 KI
8.97		 KNO3
9.69
--->
 - - - - - H3BO3
0.39		 CaI2
3.54		 Ca(NO3)2
9.27
--->
 - - - - - KBr
0.75		 - -

* Add NaHCO3 last.

Return to list of Table 3 subdivisions

TABLE 3B. ASWs #9-15: Additional Ca++-free and buffer variations (see KEY)

Values shown = millimolar (mM) concentration = ml 1M stock solution per liter
To determine grams per liter, see Calculations (App. 1) and Formula Weights (App. 2).

         ASW# --->
Ingredients		 9
CaF-M		 10
CaF-S		 11
CaF-E		 12
NaF		 13
TES-NaF		 14
TES		 15
MOPS
NaCl 436.00 442.50 439.00 - - 425.00 460.00
KCl 9.68 9.83 8.90 4.00 9.40 9.40 10.00
CaCl2 - - - - 9.00 9.00 10.00
MgCl2 34.13 24.58 23.00 - 22.10 22.10 25.00
MgSO4 16.67 16.80 22.50 - 25.60 25.60 27.00
*NaHCO3 - - 4.60 - - 2.10 2.30
H2O to 1L to 1L to 1L to 1L to 1L to 1L to 1L
Final pH - - 7.62
with NaOH		 8.3
with KOH		 7.9
with KOH		 7.9
with KOH		 7.5
Other Ingred. 9 10 11 12 13 14 15
--->
 - - EGTA
1.00		 KHCO3
5.40		 TES
10.00		 TES
10.00		 MOPS
5.00
--->
 - - - choline
chloride
455.40		 choline
chloride
425.00		 - -
--->
 - - - - KHCO3
2.10		 - -

* Add NaHCO3 last.

Return to list of Table 3 subdivisions

TABLE 3C.   ASWs #16-22: Some older ASW vintages (see KEY)

Values shown = millimolar (mM) concentration = ml 1M stock solution per liter
To determine grams per liter, see Calculations (App. 1) and Formula Weights (App. 2).

         ASW#---->
Ingredients		 16
BZ		 17
AP		 18
HB		 19
HR		 20
VHa		 21
VHb		 22
CH
NaCl 419.90 423.00 430.50 428.00 430.50 432.00 395.00
KCl 9.00 9.10 8.73 9.80 9.40 9.54 8.88
CaCl2 9.50 9.50 9.76 9.97 7.22 4.36 21.15
MgCl2 23.75 23.50 28.36 29.35 34.31 33.78 31.36
MgSO4 25.38 25.53 18.35 18.07 16.52 16.52 16.78
*NaHCO3 2.23 1.15 2.18 2.38 "trace" - -
H2O to 1L to 1L to 1L to 1L to 1L to 1L to 1L
Final pH - - - - - - -
Other Ingred. 16 17 18 19 20 21 22
--->
 NaBr
0.75		 - - - - - MgBr2
0.38
--->
 - - - - - - Na2CO3
10.94

*Add NaHCO3 last, just before using

Return to list of Table 3 subdivisions